[英文釋義] The transfer of genetic information from DNA to messenger RNA by DNA-directed RNA polymerase. It includes reverse transcription and transcription of early and late genes expressed early in an organism's life cycle or during later development.
reverse vt. 1.使顛倒,使倒轉(zhuǎn),使反轉(zhuǎn);使翻轉(zhuǎn);翻(案)。 2.掉換,交換。 3.使成正相反的東西,完全改變。 4.【機(jī)械工程】使倒退,使繞行,使倒開。 5.【法律】撤消,推翻。 reverse an engine 倒車。 reverse a motion [policy] 使運(yùn)動(dòng)倒轉(zhuǎn)[完全改變政策]。 reverse an order 顛倒次序。 reverse positions 掉換位置。 R- arms! 倒槍!〔行葬禮時(shí)使槍口向地的命令〕。 a reversed line 【物理學(xué)】自蝕(光線)譜。 reverse the verdict 翻案。 reverse the charge 讓接電話的人付電話費(fèi)。 reverse oneself 完全改變自己主張,使逆轉(zhuǎn)。 vi. 顛倒,倒轉(zhuǎn),反轉(zhuǎn),倒退;逆轉(zhuǎn);反向;【機(jī)械工程】回動(dòng);(跳舞時(shí))向左轉(zhuǎn)。 adj. 1.反對(duì)的,相反的,倒轉(zhuǎn)的,顛倒的;翻轉(zhuǎn)的,反面的,背后的;朝后的;反卷的;反向的,倒開的,回動(dòng)的,逆流的。 2.【生物學(xué)】倒卷的,左卷的。 in the reverse direction 朝著相反的方向。 in reverse order 次序顛倒地。 n. 1.反對(duì),相反。 2.(硬幣等的)反面,背面 (opp. obverse)。 3.倒轉(zhuǎn),顛倒;反向;【機(jī)械工程】回動(dòng);回動(dòng)裝置[齒輪]。 4.(槍等的)托尾。 5.逆境,倒霉,不幸,挫折,失敗。 6.【劍術(shù)】倒擊,倒刺。 7.【跳舞】左轉(zhuǎn)。 the very reverse 正相反。 the reverses of fortune 運(yùn)氣不佳;災(zāi)難,不幸。 With others the reverse (of this) happens. 其他各人遭遇完全相反。 in reverse 相反;【軍事】在陣后,在[從]背面。 meet with reverses 遭受挫折;倒霉;失??;吃敗仗。 on the reverse (汽車)倒開著。 quite the reverse 正相反。 suffer [sustain, have] a reverse 遭受失??;被打敗,吃敗仗。 take in reverse 從背面攻擊。 adj. -d 顛倒的;撤銷了的。 adv. =reversely 顛倒地,翻轉(zhuǎn)地,反對(duì)地,(和這)相反地,在另一方面。
Protocol of reverse transcription polymerase chain reaction rt-pcr for infectious haematopoietic necrosis virus 傳染性造血器官壞死病毒逆轉(zhuǎn)錄聚合酶鏈?zhǔn)椒磻?yīng)操作規(guī)程
Influenza virus sample in cultured cells was treated for reverse transcription, amplification, fluorescent dye labelling, hybridization and scanning 對(duì)待檢樣品進(jìn)行隨機(jī)逆轉(zhuǎn)錄和擴(kuò)增,熒光標(biāo)記,雜交,掃描分析。
Pgem-t vector system, jm109 competent cells, reverse transcription kit and in vitro translation kit were purchased from promega company pgem一t載體,感受態(tài)細(xì)菌jm109,反轉(zhuǎn)錄試劑盒和體外翻譯試劑盒均購(gòu)自promega公司。
The ghr cdna was isolated from southern catfish ( silurus meridionalis ) by reverse transcription polymerase chain reaction followed by rapid amplification of cdna ends 用rt-per加race的方法從南方大口鲇中克隆出其生長(zhǎng)激素受體(ghr)。
The nucleoprotein ( n ) gene of three strains and glycoprotein ( g ) gene of 119, gxbm were amplified by reverse transcription-polymerase chain reaction ( rt-pcr ), respectively 對(duì)三株的n基因和119、gxbm株的g基因進(jìn)行了rt-pcr擴(kuò)增、克隆和測(cè)序。
Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5-dctp or cy3-dctp into the reaction during reverse transcription of rna 采用cartesian基因芯片打印儀,將純化的cdna片段打印在corning玻片表面,制作了酵母細(xì)胞基因表達(dá)譜芯片。
First, mrna was purified from the total rna extracted from fresh spleens of nonimmunized mice of kunming, then the cdna library was achieved via reverse transcription pcr . gene fragments encoding v 首先從未免疫小鼠的脾臟中提取總rna,分離出mrna,經(jīng)逆轉(zhuǎn)錄合成其總cdna。
The cdna encoding growth hormone ( gh ) peptide was amplified by reverse transcription polymerase chain reaction ( rt-pcr ) method using isolated total rna as template 應(yīng)用逆轉(zhuǎn)錄-聚合酶鏈?zhǔn)椒磻?yīng)(rt-pcr)技術(shù)克隆得到編碼草魚生長(zhǎng)激素(cgh)的基因cdna,并定向克隆到puc18載體上。
Viral rnas were extracted from virus-infected allantoic fluids using qiaamp mini-extraction kits . after reverse transcription, cdna was amplified using specific primers for each gene segment 用qiagen的rneasyrna試劑盒提取病毒rna后,逆助興才李醫(yī)‘筍脂碩圣‘老‘才轉(zhuǎn)錄合成cdna。